Anti C4d
Produktdaten
| Product name | Anti C4d |
| Cat-Nr. | BI-RC4D |
| Sample volume | 250 μl |
| Sample type | paraffin embedded tissue sections, frozen sections |
| Species | Human |
| Tests | 30-50 |
| Method | Immunohistochemistry |
Intended use
The antibody has been tested for use on paraffin sections and frozen sections of human tissue. Working protocol available. Recommended dilution for immunohistochemistry: 1:30 – 1:50 Pretreatment of slides by pressure cooking (5 minutes at +125°C in TRIS/EDTA buffer pH 8.5) is highly recommended for antigen retrieval.
Intended use:
C4d is the degradation product of the activated complement factor C4, a component of the classical complement cascade, which is typically initiated by binding of antibodies to specific target molecules. Detection of C4d is regarded as an indirect sign, a "footprint" of an antibody response against the allograft. The majority of publications describe C4d as an important biomarker in kidney transplantation but also in heart, liver, and other transplants.
Intended applications:
- Humoral transplant rejection
